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抗生素 3

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农田土壤 1

制药工业 1

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Nitrifying and denitrifying bacteria in aerobic granules formed in sequencing batch airlift reactors

WANG Fang, YANG Fenglin, QI Aijiu

《环境科学与工程前沿（英文）》 2007年 第1卷 第2期   页码 184-189 doi: 10.1007/s11783-007-0032-2

摘要： The purpose of this study was to investigate nitrifying bacteria and denitrifying bacteria isolated from aerobic granules. Aerobic granules were formed in an internal-circulate sequencing batch airlift reactor (SBAR) and biodegradation of NH3--N was analyzed in the reactor. Bacteria were isolated and determined from aerobic granules using selected media. The growth properties and morphology of bacteria colonies were observed by controlling aerobic or anaerobic conditions in the culture medium. It was found that bacteria in aerobic granules were diverse and some of them were facultative aerobes. The diversity of bacteria in aerobic granules was a premise of simultaneous nitrification and denitrification.
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LYX: A novel aerobic bacterium with advantage of removing nitrate high effectively by assimilation and

Yuxin Li, Jiayin Ling, Pengcheng Chen, Jinliang Chen, Ruizhi Dai, Jinsong Liao, Jiejing Yu, Yanbin Xu

《环境科学与工程前沿（英文）》 2021年 第15卷 第4期 doi: 10.1007/s11783-020-1349-3

摘要： Abstract • Pseudomonas mendocina was first reported for aerobic nitrate removal. • It removed 90% of NO3−-N in 24 h under aerobic conditions. • This strain converted NO3−-N to bio-nitrogen (37.9%) and gaseous nitrogen (49.7%). • Inoculation of this strain increased sludge denitrification rate by 4.3 times. The problem of nitrate accumulation in aerobic tank and total nitrogen excessive discharge in effluent was very common in traditional livestock and poultry farming wastewater treatment systems owing to the lengthy process flow and low process control level. A strain LYX of aerobic bacterium was isolated from the activated sludge of a wastewater treatment system in a pig farm, which could remove nitrate effectively in aerobic tank and was identified Pseudomonas mendocina by 16S rRNA sequencing. Under the condition of nitrate as the sole nitrogen source, this strain removed over 90% of NO3−-N with an initial concentration of 110 mg/L under aerobic conditions within 48 hours. Among them, 37.9% of NO3−-N was assimilated into Bio-N, about 51.9% was reduced to gaseous nitrogen and less than 0.5% of nitrogen was replaced by NO2−-N and NH4+-N, 9.7% NO3−-N remained in the effluent at the end. At the same time, four key genes (napA, nirK, norB and nosZ) related to nitrate nitrogen removal were expressed during the denitrification process of P. mendocina LYX, in which the transcription level of the indicator genes of this aerobic denitrifying bacterium (napA) was the highest. In addition, it was found with the 15N tracer technique that inoculation of this strain on sludge increased the amount of nitrogen loss from 9.26 nmol N/(g·h) to 23.835 nmol N/(g·h). Therefore, P. medocina LYX is a potential bioagent for advanced nitrogen removal by assimilating and reducing nitrate simultaneously in aerobic tanks.

关键词： Pseudomonas mendocina     Aerobic nitrate removal     15N tracing technique     Denitrification assimilatively and disimilatively     Aerobic denitrifying genes    

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Environmental effects and risk control of antibiotic resistance genes in the organic solid waste aerobic

《环境科学与工程前沿（英文）》 2021年 第15卷 第6期 doi: 10.1007/s11783-021-1415-5

摘要：

• ARGs were detected in livestock manure, sludge, food waste and fermentation dregs.

关键词： Antibiotic resistance genes     Organic solid waste     Aerobic composting     Livestock manure     Sludge    

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Influence of hydraulic retention time on behavior of antibiotics and antibiotic resistance genes in aerobic

Jie Liao, Chaoxiang Liu, Lin Liu, Jie Li, Hongyong Fan, Jiaqi Ye, Zhichao Zeng

《环境科学与工程前沿（英文）》 2019年 第13卷 第3期 doi: 10.1007/s11783-019-1115-6

摘要：

Longer HRT can enhance degradation rate of sulfamethoxazole in granular reactor.

Longer HRT can reduce accumulated concentrations of TCs and QNs in sludge.

Longer HRT may have increased relative abundances of ARGs in aerobic granules.

关键词： Aerobic granules     Swine wastewater     Tetracyclines     Fluoroquinolones     Sulfonamides    

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MB（A2/O）工艺的好氧、缺氧摄磷及微生物特性

吴剑,王世和

《中国工程科学》 2007年 第9卷 第11期   页码 191-195

摘要：

为开发高效除磷脱氮技术，研制了将MBR与A2/O工艺有机集成的新型MB（A2/O）组合工艺。研究 了MB（A2/O）工艺处理城市污水的好氧、缺氧摄磷性能及微生物特性，并分析了其机理。结果表明：在 MB（A2/O）系统中，聚磷菌约占活性污泥总量的20%~40%，其中大量存在能够利用NOX－N作为电子受体 进行反硝化除磷的DPB，约占聚磷菌数量的35. 66%~67. 83%。好氧摄磷的平均速率为2. 30mgP·gMLSS-1·h-1，最大摄磷速率为5. 44mgP·gMLSS-1·h-1；缺氧摄磷的平均速率为1. 56 mgP·gMLSS-1·h-1，最大摄磷速率为1. 94 mgP·gMLSS-1·h-1。

关键词： 好氧摄磷     反硝化除磷     聚磷菌     电子受体    

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Effect of different carbon sources on performance of an A

Dongliang Du, Chuanyi Zhang, Kuixia Zhao, Guangrong Sun, Siqi Zou, Limei Yuan, Shilong He

《环境科学与工程前沿（英文）》 2018年 第12卷 第2期 doi: 10.1007/s11783-017-0981-z

摘要： Effect of different carbon sources on purification performance and change of microbial community structure in a novel A N-MBR process were investigated. The results showed that when fed with acetate, propionate or acetate and propionate mixed (1:1) as carbon sources, the effluent COD, NH -N, TN and TP were lower than 30, 5, 15 and 0.5 mg·L , respectively. However, taken glucose as carbon source, the TP concentration of effluent reached 2.6 mg·L . Process analysis found that the amount of anaerobic phosphorus release would be the key factor to determine the above effectiveness. The acetate was beneficial to the growth of associated with biological phosphorus removal, which was the main cause of high efficiency phosphorus removal in this system. In addition, it could eliminate the associated with glycogen-accumulating organisms and guarantee high efficiency phosphorus uptake of phosphorus accumulating organisms in the system with acetate as carbon source.

关键词： Denitrifying phosphorus removal     Alternate anaerobic/anoxic-aerobic MBR (A2N-MBR)     Carbon source     Operation characteristic     Community structure    

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Effect of denitrifying bacteria on the electrochemical reaction of activated carbon fiber in electrochemical

YING Diwen, JIA Jinping, ZHANG Lehua

《环境科学与工程前沿（英文）》 2007年 第1卷 第3期   页码 305-310 doi: 10.1007/s11783-007-0051-z

摘要： An electrochemical-activated denitrifying biofilm system consisting of activated carbon fiber electrodes immobilized with denitrifying bacteria film as cathode was studied. A revised model for an electrochemical-activated denitrifying biofilm was developed and validated by electrochemical analysis of cathodal polarization curves and nitrate consumption rate. The cathodal polarization curve and nitrate consumption rate were introduced to verify the rate of electrochemical reaction and the activity of denitrifying bacteria, respectively. It was shown that the denitrification process effectively strengthened the electrochemical reaction while the electron also intensified denitrification activity. Electron was transferred between electrochemical process and biological process not only by hydrogen molecule but also by new produced active hydrogen atom. Additionally, a parameter of apparent exchange current density was deprived from the cathodal polarization curve with high overpotential, and a new bio-effect current density was defined through statistical analysis, which was linearly dependent to the activity of denitrification bacteria. Activated carbon fiber (ACF) electrode was also found to be more suitable to the electrochemical denitrifying system compared with graphite and platinum.

关键词： ACF     apparent exchange     hydrogen molecule     activated     biological process    

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Toxic effect of sodium perfluorononyloxy-benzenesulfonate on in aerobic denitrification, cell structure

《环境科学与工程前沿（英文）》 2021年 第15卷 第5期 doi: 10.1007/s11783-021-1391-9

摘要：

• OBS inhibited the growth of P. stutzeri and destroyed its structure.

关键词： Sodium perfluorononyloxy-benzenesulfonate     Aerobic denitrification     Pseudomonas stutzeri     Ecotoxicity     ROS     Persist organic pollutants     Toxicity     Denitrification     Microbiology    

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Optimization of thermophilic anaerobic-aerobic treatment system for Palm Oil Mill Effluent (POME)

Yijing CHAN,Meifong CHONG,Chunglim LAW

《环境科学与工程前沿（英文）》 2015年 第9卷 第2期   页码 334-351 doi: 10.1007/s11783-014-0626-4

摘要： Optimization of an integrated anaerobic-aerobic bioreactor (IAAB) treatment system for the reduction of organic matter (Chemical Oxygen Demand (COD), Biochemical Oxygen Demand (BOD) and Total Suspended Solids (TSS) concentrations) in Palm Oil Mill Effluent (POME) to legal standards with high methane yield was performed for the first time under thermophilic condition (50°C–55°C) by using response surface methodology (RSM). The experiments were conducted based on a central composite rotatable design (CCRD) with three independent operating variables, organic loading rates in anaerobic compartment (OLR ) and mixed liquor volatile suspended solids (MLVSS) concentration in anaerobic (MLVSS ) and aerobic compartments (MLVSS ). The optimum conditions for the POME treatment were determined as OLR of 15.6 g COD·L ·d , MLVSS of 43100 mg·L , and MLVSS of 18600 mg·L , where high aerobic COD, BOD and TSS removal efficiencies of 96.3%, 97.9%, and 98.5% were achieved with treated BOD of 56 mg·L and TSS of 28 mg·L meeting the discharge standard. This optimization study successfully achieved a reduction of 42% in the BOD concentrations of the final treated effluent at a 48% higher OLR as compared to the previous works. Besides, thermophilic IAAB system scores better feasibility and higher effectiveness as compared to the optimized mesophilic system. This is due to its higher ability to handle high OLR with higher overall treatment efficiencies (more than 99.6%), methane yield (0.31 L CH ·g COD ) and purity of methane (67.5%). Hence, these advantages ascertain the applicability of thermophilic IAAB in the POME treatment or even in other high-strength wastewaters treatment.

关键词： palm oil mill effluent (POME)     anaerobic     aerobic     thermophilic     biogas     optimization    

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Online control of biofilm and reducing carbon dosage in denitrifying biofilter: pilot and full-scale

Xiuhong Liu, Hongchen Wang, Qing Yang, Jianmin Li, Yuankai Zhang, Yongzhen Peng

《环境科学与工程前沿（英文）》 2017年 第11卷 第1期 doi: 10.1007/s11783-017-0895-9

摘要： Online control of DNBF was studied in the pilot-scale and full-scale experiments. DNBF was controlled by the online monitored effluent nitrate and turbidity. The effluent nitrate lower than 3 mg·L and saving 18% of carbon were both achieved. Denitrifying biofilter (DNBF) is widely used for advanced nitrogen removal in the reclaimed wastewater treatment plants (RWWTPs). Manual control of DNBF easily led to unstable process performance and high cost. Consequently, there is a need to automatic control of two decisive operational processes, carbon dosage and backwash, in DNBF. In this study, online control of DNBF was investigated in the pilot-scale DNBF (600 m ·d ), and then applied in the full-scale DNBF (10 × 104 m3·d ). A novel simple online control strategy for carbon dosage with the effluent nitrate as the sole control parameter was designed and tested in the pilot-scale DNBF. Backwash operation was optimized based on the backwash control strategy using turbidity as control parameter. Using the integrated control strategy, in the pilot-scale DNBF, highly efficient nitrate removal with effluent TN level lower than 3 mg·L was achieved and DNBF was not clogged any more. The online control strategy for carbon dosage was successfully applied in a RWWTP. Using the online control strategy, the effluent nitrate concentration was controlled relatively stable and carbon dosage was saved for 18%.

关键词： Reclaimed water treatment     Denitrifying biofilter     Carbon dosage     Backwash control    

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Metagenomic analysis on resistance genes in water and microplastics from a mariculture system

《环境科学与工程前沿（英文）》 2022年 第16卷 第1期   页码 4-4 doi: 10.1007/s11783-021-1438-y

摘要：

Microplastics existing widely in different matrices have been regarded as a reservoir for emerging contaminants. Mariculture systems have been observed to host microplastics and antibiotic resistance genes (ARGs). However, more information on proliferation of ARGs and metal resistance genes (MRGs) in mariculture system at the presence of microplastics is needed.

关键词： Antibiotic resistance genes     Metal resistance genes     Metagenomic analysis     Microplastics     Mariculture    

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Characteristics of night soil and leaf co-composting using aerobic static method

Xiaojie SUN, Dunqiu WANG, Wenjing LU, Hongtao WANG

《环境科学与工程前沿（英文）》 2012年 第6卷 第3期   页码 421-427 doi: 10.1007/s11783-011-0380-9

摘要： The main purpose of this work is to investigate the characteristics of night soil and leaf co-composting using aerobic static composting method. Three influencing factors, including proportion of night soil and leaf, aeration rate and aeration pattern, were investigated through the evolution of the principal physicochemical properties, i.e., temperature, oxygen consumption rate, organic matters, moisture content, carbon, nitrogen, carbon-to-nitrogen ratio and Germination index (GI). It was found that the 3∶1 (w∶w) mixture of night soil and leaf was capable of achieving the highest composting temperature, longest retention time of high temperature (55°C), and fastest organic matter degradation. The 0.14 m ·min ·m aeration rate was most beneficial to composting, and the mixture of night soil and leaf maintained the highest temperature for the longest duration and achieved the highest CO content and GI. The continuous aeration pattern during composting was superior to an intermittent aeration pattern, since the latter delayed the composting process.

关键词： night soil     leaf     aerobic compost     aeration rate     aeration pattern    

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Scaling up a novel denitrifying microbial fuel cell with an oxic-anoxic two stage biocathode

Peng LIANG, Jincheng WEI, Ming LI, Xia HUANG

《环境科学与工程前沿（英文）》 2013年 第7卷 第6期   页码 913-919 doi: 10.1007/s11783-013-0583-3

摘要： A scaled up microbial fuel cell (MFC) of a 50 L volume was set up with an oxic-anoxic two-stage biocathode and activated semicoke packed electrodes to achieve simultaneous power generation and nitrogen and organic matter removals. An average maximum power density of 43.1 W·m was obtained in batch operating mode. By adjusting the two external resistances, the denitrification in the A-MFC and power production in the O-MFC could be enhanced. In continuous mode, when the hydraulic retention times were set at 6 h, 8 h and 12 h, the removal efficiencies of COD, and total nitrogen (TN) were higher than 95%, 97%, and 84%, respectively. Meanwhile the removal loads for COD, and TN were10, 0.37 and 0.4 kg·(m ·d) , respectively.

关键词： microbial fuel cell (MFC)     oxic-anoxic two stage biocathode     denitrifying    

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Aerobic granulation of pure bacterial strain

ADAV Sunil S., LEE Duu-Jong

《环境科学与工程前沿（英文）》 2008年 第2卷 第4期   页码 461-467 doi: 10.1007/s11783-008-0066-0

摘要： The objective of this study is to cultivate aerobic granules by pure bacterial strain, , in a sequencing batch reactor. Stable granules sized 2.0–2.2 mm were formed in the reactor after a five-week cultivation. These granules exhibited excellent settling attributes, and degraded phenol at rates of 1.49 and 1.19 g phenol/(g VSS·d) at 250 and 1500 mg/L of phenol concentration, respectively. Confocal laser scanning microscopic test results show that was distributed over the initial small aggregates, and the outer edge of the granule was away from the core regime in the following stage.

关键词： following     excellent settling     phenol/     sequencing     microscopic    

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Nitrogen-retaining property of compost in an aerobic thermophilic composting reactor for the sanitary

Fan BAI, Xiaochang WANG,

《环境科学与工程前沿（英文）》 2010年 第4卷 第2期   页码 228-234 doi: 10.1007/s11783-010-0022-7

摘要： Aerobic composting is a method for the sanitary disposal of human feces as is used in bio-toilet systems. As the products of composting can be utilized as a fertilizer, it would be beneficial if the composting conditions could be more precisely controlled for the retention of fecal nitrogen as long as possible in the compost. In this study, batch experiments were conducted using a closed aerobic thermophilic composting reactor with sawdust as the bulk matrix to simulate the condition of a bio-toilet for the sanitary disposal of human feces. Attention was paid to the characteristics of nitrogen transformation. Under the controlled conditions of temperature at 60°C, moisture content at 60%, and a continuous air supply, more than 70% fecal organic removal was obtained, while merely 17% fecal nitrogen loss was observed over a two-week composting period. The nitrogen loss was found to occur mainly in the first 24 h with the rapid depletion of inorganic nitrogen but with an almost unchanged organic nitrogen content. The fecal NH–N which was the main component of the inorganic nitrogen (>90%) decreased rapidly in the first day, decreased at a slower rate over the following days, and finally disappeared entirely. The depletion of NH–N was accompanied by the accumulation of NH gas in the ammonia absorber connected to the reactor. A mass balance between the exhausted NH gas and the fecal NH–N content in the first 24 hours indicated that the conversion of ammonium into gaseous ammonia was the main reason for nitrogen loss. Thermophilic composting could be considered as a way to keep a high organic nitrogen content in the compost for better utilization as a fertilizer.

关键词： nitrogen retention     composting reactor     human feces     aerobic     thermophilic     fertilizer    

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标题 作者 时间 类型 操作

Nitrifying and denitrifying bacteria in aerobic granules formed in sequencing batch airlift reactors

WANG Fang, YANG Fenglin, QI Aijiu

期刊论文

LYX: A novel aerobic bacterium with advantage of removing nitrate high effectively by assimilation and

Yuxin Li, Jiayin Ling, Pengcheng Chen, Jinliang Chen, Ruizhi Dai, Jinsong Liao, Jiejing Yu, Yanbin Xu

期刊论文

Environmental effects and risk control of antibiotic resistance genes in the organic solid waste aerobic

期刊论文

Influence of hydraulic retention time on behavior of antibiotics and antibiotic resistance genes in aerobic

Jie Liao, Chaoxiang Liu, Lin Liu, Jie Li, Hongyong Fan, Jiaqi Ye, Zhichao Zeng

期刊论文

MB（A2/O）工艺的好氧、缺氧摄磷及微生物特性

吴剑,王世和

期刊论文

Effect of different carbon sources on performance of an A

Dongliang Du, Chuanyi Zhang, Kuixia Zhao, Guangrong Sun, Siqi Zou, Limei Yuan, Shilong He

期刊论文

Effect of denitrifying bacteria on the electrochemical reaction of activated carbon fiber in electrochemical

YING Diwen, JIA Jinping, ZHANG Lehua

期刊论文

Toxic effect of sodium perfluorononyloxy-benzenesulfonate on in aerobic denitrification, cell structure

期刊论文

Optimization of thermophilic anaerobic-aerobic treatment system for Palm Oil Mill Effluent (POME)

Yijing CHAN,Meifong CHONG,Chunglim LAW

期刊论文

Online control of biofilm and reducing carbon dosage in denitrifying biofilter: pilot and full-scale

Xiuhong Liu, Hongchen Wang, Qing Yang, Jianmin Li, Yuankai Zhang, Yongzhen Peng

期刊论文

Metagenomic analysis on resistance genes in water and microplastics from a mariculture system

期刊论文

Characteristics of night soil and leaf co-composting using aerobic static method

Xiaojie SUN, Dunqiu WANG, Wenjing LU, Hongtao WANG

期刊论文

Scaling up a novel denitrifying microbial fuel cell with an oxic-anoxic two stage biocathode

Peng LIANG, Jincheng WEI, Ming LI, Xia HUANG

期刊论文

Aerobic granulation of pure bacterial strain

ADAV Sunil S., LEE Duu-Jong

期刊论文

Nitrogen-retaining property of compost in an aerobic thermophilic composting reactor for the sanitary

Fan BAI, Xiaochang WANG,

期刊论文